quantikine elisa  (R&D Systems)

Journal: Stem Cell Research & Therapy

Article Title: IL-1β modulates inflammatory response of human bone marrow-derived MSCs and neutrophil recruitment in vitro via NF-kB-associated signaling

doi: 10.1186/s13287-026-05029-x

Figure Lengend Snippet: IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or ELISA. Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001

Article Snippet: CXCL1 was measured in diluted 1:1 or undiluted CM samples using Quantikine ELISA (cat# DGR00B, R&D Systems).

Techniques: Expressing, Control, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: bioRxiv

Article Title: Soluble E-cadherin Drives Brain Metastasis in Inflammatory Breast Cancer

doi: 10.1101/2025.06.18.660428

Figure Lengend Snippet: (A, B) Cytokine array analysis of secreted factors showed increased levels of the pro-inflammatory cytokines CXCL1/CXCL8 in conditioned medium from sEcad-overexpressing MDA-IBC3 cells, as validated by ELISA. (C) CXCL1 was expressed at higher levels in patients with brain metastasis than in patients with metastasis to other anatomic sites. (D) Patients with high CXCL1 expression had reduced brain metastasis–free survival (BrMFs, (GSE12276); CXCL1-low indicates the bottom (25th) tertile, and CXCL1-high the top (75th) tertile. (E) CXCL8 was expressed at higher levels in patients with brain metastasis relative to patients with metastasis to other anatomic sites. (F) Patients with high CXCL8 expression had reduced brain metastasis – free survival (BrMFs, (GSE12276); CXCL8-low indicates the bottom (25th) tertile, and CXCL8-high the top (75th) tertile. (G) CXCR2 was expressed at higher levels in patients with brain metastasis than in patients with metastasis to other anatomic sites. (H) Patients with high CXCR2 expression had reduced brain metastasis–free survival (BrMFs, (GSE12276); CXCL2-low indicates the bottom (25th) tertile, and CXCL2-high the top (75th) tertile. (I) CXCR2 expression was induced in sEcad-overexpressing MDA-IBC3 and SUM149 cells relative to control cells. (J, K) Induction of CXCR2 expression in human astrocytes (J) and mouse astrocytes (K) treated with 30% condition medium from sEcad-overexpressing cells. GAPDH served as a loading control.

Article Snippet: For conditioned-medium ELISA, cancer cells were cultured with serum-free medium for 36 h, after which the conditioned medium was collected and subjected to ELISA for CXCL1, CXCL8 and DKK1 (R&D Systems, Minneapolis, MN, USA #DGR00B, #D8000C, and #DKK100B, respectively).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Control

Journal: PLOS ONE

Article Title: Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

doi: 10.1371/journal.pone.0313693

Figure Lengend Snippet: Proteins secreted from the medium were analyzed using a RayBio Biotin Label-based Human Antibody Array. (A) Increased spot intensity for GRO-α was observed in cells derived from the same donor. The selection of intersecting proteins among candidate proteins secreted by hWJ-MSCs was higher than that of hPL-MSCs. (B) The conditioned media was collected from C2C12, hWJ-MSCs alone, hPL-MSCs alone, and co-cultured cells. The concentration of secreted GRO-α in each conditioned medium was measured using the GRO-α ELISA kit (** p < 0.01, * p < 0.05, n = 4). Data are significantly different from that in the corresponding control group.

Article Snippet: The Human GRO-α Quantikine ELISA kit (#DGR00B; R&D systems, USA) was used to analyze the expression of proteins selected from the antibody array.

Techniques: Ab Array, Derivative Assay, Selection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: PLOS ONE

Article Title: Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

doi: 10.1371/journal.pone.0313693

Figure Lengend Snippet: In vitro damaged C2C12 cells were co-cultured with hWJ-MSCs pretreated with GRO-α siRNAs, which reduced the levels of secreted GRO-α. (A) To examine knockdown of GRO-α in hWJ-MSCs, the medium was collected and GRO-α levels were analyzed using ELISA (*** p <0.001, n = 3). (B) Harvested cells were analyzed through western blotting with specific antibodies (p-RIP3, p-MLKL, β-actin). (C, D) All bands were analyzed through densitometry (** p <0.01, * p < 0.05, n = 3).

Article Snippet: The Human GRO-α Quantikine ELISA kit (#DGR00B; R&D systems, USA) was used to analyze the expression of proteins selected from the antibody array.

Techniques: In Vitro, Cell Culture, Knockdown, Enzyme-linked Immunosorbent Assay, Western Blot